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Re: first shed...
Hans,
It really is a dangerous game to play if you're primarily feeding fish that contain the thiaminase enzyme since one enzyme molecule can process tens of thousands (maybe more) substrate molecules during its "lifetime." You're exactly right about it being a matter of density between the enzyme and substrate concentrations, but since you can't easily see the effects until it's too late, it's hard to know if you're giving too much or too little thiamine supplement.
The effects of feeding a diet of only goldfish without B1 supplementation, for example, may take 6 months or longer to appear. Then, they could easily be mistaken for general illness or stress. Low level thiamine deficiencies may only result in decreased growth rate or reproductive failure, rather than weakness or death, but who wants either of those to happen? There are so many other choices of nutritious fish out there, that I think the risks involved outweigh the need to feed fish that contain thiaminase.
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